Which of the following molecule is preferred for the radioactive Labelling using PNK?

T4 PNK, a specialized enzyme, holds a pivotal role in the realm of molecular biology. Its primary application revolves around the precise labeling of polynucleotides, which are long chains of nucleotides that form the backbone of DNA and RNA.

This labeling process involves the attachment of radioactive phosphate groups to the 5′ ends of these polynucleotides. The source of these radioactive phosphate groups is ATP, a crucial energy-carrying molecule in cells, that has been modified with an isotope to impart radioactivity.

The utilization of T4 PNK in this context is advantageous due to its high specificity and efficiency in catalyzing the transfer of the radioactive phosphate from the modified ATP to the target polynucleotide. This ensures accurate and reliable labeling for subsequent experimental procedures.

The labeled polynucleotides can then be employed in various research endeavors, including the study of DNA replication, repair, and transcription processes. They also find applications in diagnostic assays and therapeutic interventions, where their unique properties enable sensitive and specific detection of target molecules.

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