Is you're welcome sarcastic?

Excuse me, I must ask, is the phrase "you're welcome" intended to be sarcastic in this context? It's a common expression used to politely acknowledge someone's thanks, but I've noticed that it can sometimes be misconstrued or used sarcastically. Could you clarify your intentions behind using it? I'd like to ensure that we're communicating effectively and that no misunderstandings arise. Thank you for your attention to this matter.